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KMID : 0364820180540030266
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Korean Journal of Microbiology
2018 Volume.54 No. 3 p.266 ~ p.271
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Effect of plasmid curing on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W
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Kim Kyoung-Ja
Lee Jae-Rim
Yang Yong-Joon
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Abstract
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Effect of plasmid curing of Acinetobacter sp. B-W on theproduction of siderophore from glutamic acid as both carbonand nitrogen sole sources was investigated. Plasmid cured mutantof strain B-W lost the ability to produce siderophore fromglutamic acid at 28°C. Transformant E. coli DH5α harboring20 kb plasmid, that was isolated from wild type of strain B-Wproduced siderophore from glutamic acid as both carbon andnitrogen sole sources at 28°C, but, not at 36°C. Production ofsiderophore from glutamic acid by transformant E. coli DH5αwas completely inhibited by 10 μM FeCl3. In previous report,catechol nature of siderophore produced from glutamic acid bystrain B-W was detected by Arnow test. The siderophoreproduced from glutamic acid by transformant E. coli DH5αwas also catechol type. Rf value of siderophore produced fromtransformant E. coli DH5α grown in medium glutamic acid asboth carbon and nitrogen sole sources at 28°C was 0.32 inbutanol-acetic acid-water (12:3:5) as developing solvent. Rfvalue of the siderophore was the same with that of wild type ofstrain B-W. Thus a single plasmid of 20 kb seemed to beinvolved in the production of siderophore from glutamic acid.
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KEYWORD
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Acinetobacter sp. B-W , L-glutamic acid , plasmid curing , siderophore , sole carbon and nitrogen sources
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